Economical Appraisal of Total Aflatoxin Level in the Poultry Feeds by Fourier Transform Infrared Spectroscopy

Single-bounce attenuated total reflectance (SB-ATR) Fourier transform infrared (FTIR) spectroscopy has been used for the quantitative determination of total aflatoxins in the broiler poultry feed. An FTIR calibration spanning the range 1-70 μg/L aflatoxin standards in (70:30, v/v) methanol-water solvent system based on partial least square (PLS) model, developed by relating mid IR region between 3755-950 cm. The excellent coefficient of determination (R = 0.998) was achieved with 1.49 relative mean square error of calibration (RMSEC). Aflatoxins from each of eight poultry feeds was extracted and the determined by the widely used commercially available Enzyme-linked Immunosorbent Assay (ELISA) procedure and the SB-ATR/FTIR method. The SBATR/FTIR aflatoxins predictions were related to those determined by the ELISA method by linear regression, producing an R value of 0.989 and a SD of ± 2.80 μg/L. The result of the study clearly indicated that FT-IR spectroscopy due to its rapidity and simplicity along with data manipulation by advance computer software could be effectively used for routine determination of aflatoxins present in the poultry feeds at very low level.